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KIDNEY FUNCTIONS IN WISTER RATS TREATED WTH ARTESUNATE AND AMODIAQUINE

Adeyomoye O.I, Adeleye A.S.

 

ABSTRACT: Malaria is a mosquito bone disease caused by plasmodium parasite. Several drugs have been developed and used in treatment of this disease but, many of these medications results in adverse side effects. This study evaluates the effects of artesunate and amodiaquine (antimalaria agents) in renal functions of wister rats. Fifteen wister rats were divided into 3groups (n=5). Group 1 served as normal control, groups 2 and 3 were treated with 8mg/kg artesunate and 30mg/kg amodiaquine respectively for 5 days after which blood samples were collected and centrifuged to obtain serum. Blood urea nitrogen (BUN) and Creatinine (CRT) levels were determined using randox kits. Kidney histology was done using haematoxylin-eosin stain. Results were analyzed using one way ANOVA with statistical significance taken at p<0.05. BUN increased significantly in 30mg/kg amodiaquine treated rats when compared with normal control and 8mg/kg artesunate treated rats. There was also significant increase in creatinine level in 30mg/kg amodiaquine treated rats when compared with normal control and 8mg/kg artesunate treated rats. Histological sections shows glomeruli distortions in both artesunate and amodiaquine treated rats. Artesunate increased BUN while amodiaquine increased both BUN and CRT which are potentially toxic at high concentrations. Artesunate may therefore be a preferred medication over amodiaquine in malaria treatment because of its reduced side effects on kidney functions.

KEY WORDS: Malaria, artesunate, amodiaquine, medication, renal function

REFERENCES:

  1. Caraballo H. "Emergency department management of mosquito-borne illness: Malaria, dengue, and west nile virus". Emergency Medicine Practice. 2014 16(5)08-01.

  2. Feldman ATWolfe D.Tissue processing and hematoxylin and eosin staining. Methods Mol Biol. 2014. 1180:31-43.

  3. GBD Mortality and Causes of Death, Collaborators, "Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980-2015: a systematic analysis for the Global Burden of Disease Study 2015. .  (10053): 1459–1544. 

  4. Laterza O, Price C, Scott M. Cystatin C. An improved estimator of glomerular filtration rate? Clin Chem. 2002. 48:699-707.

  5. Maroni B, Steinman T, Mitch W. A method for estimating nitrogen intake of patients with chronic renal failure. Kidney Int. 1985;27:58- 65.

  6. Miltuninovic J, Cutler R, Hoover P, Meijsen B, Scribner B. Measurement of residual glomerular filtration rate in the patient receiving repetitive hemodialysis. Kidney Int. 1975; 8:185-190

  7. Nada S. Abdelwahab. Spectrophotometric methods for simultaneous determination of Carvedilol and Hydrochlorothiazide in combined dosage form. Arabian Journal of Chemistry 2016. 9; S355–S360

  8. Nair, A; Abrahamsson, B; Barends, DM; Groot, DW; Kopp, S; Polli, JE; Shah, VP; Dressman, JB. "Biowaiver monographs for immediate release solid oral dosage forms: amodiaquine hydrochloride.". Journal of pharmaceutical sciences. 2012.  (12): 4390–401. 

  9. National Institute of Health. Guide for the Care and Use of Laboratory Animals. NIH publi­cation 1985; 23 – 85.

  10. Nevill CG, Verhoeff FH, Munafu CG, Ten Hove WR, Van der Kaay HJ, Were JBO. A comparison of amodiaquine and chloroquine in the treatment therapy of falciparum malaria in Kenya. East Afr Med J. 1994. 71: 167–170.

  11. Olliaro, P; Mussano, P. "Amodiaquine for treating malaria.".  2003 (2): 33-42

  12. PapiyaBigoniya, Vikalp Tiwari. Hematological and biochemical effects of sub-chronic exposure in rats. Toxicological reports 2015.2: 280 – 288

  13. Price C, Finney H. Developments in the assessment of glomerular filtration rate. ClinicaChimicaActa. 2000 ;297(1-2):55-66.

  14. Radloff PD, Philipps J, Nkeyi M, Hutchinson D, Kremsner PG. Atovaquone and proguanil for Plasmodium falciparum malaria. Lancet 1996;347: 1511–1514.

  15. White, N.J. Delaying antimalaria drugs resistance with combination chemotherapy. , 1999; 41: 301 – 308

  16. World Malaria Report 2014. Geneva, Switzerland: World Health Organization. pp. 2016; 32–42. 

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