Observation Report

      Abstract    

        

MEASURE OF ANTIOXIDANT POTENTIAL OF PLASMA BY LC-MS/MS

Raffaele Conte, Giusy De Rosa, Andrea Cavallo, Antonio Fico

 

ABSTRACT: Liquid chromatography-triple quadrupole mass spectrometry is a technique suited for rapid analysis of multiple analytes of similar and different structures and physicochemical properties. Antioxidants are a diverse group of biologically active compounds that can be present in plasma with the aim to contrast oxidative events related with atherosclerosis, arterial hypertension, stroke, myocardial infarction, diabetes, arthritis, dementia, colitis, pancreatitis, respiratory diseases, cancer, infections, etc . Current measures of antioxidant potential of plasma are based on spettrophotometry and are not able to identify the typology of antioxidants. Then,  a sensitive and specific LC-MS/MS analytical method was developed for the identification and quantification of plasma antioxidants in order to assess the biological antioxidant potential of this matrix

KEY WORDS: Antioxidants, Liquid chromatography-mass spectrometry, Biological antioxidant potential

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