Peer Reviewed Open Access Research Journal
International Journal of Medical Laboratory Research (IJMLR)
DOI: 10.35503
Observation Report
Abstract
MEASURE OF ANTIOXIDANT POTENTIAL OF PLASMA BY LC-MS/MS
Raffaele Conte, Giusy De Rosa, Andrea Cavallo, Antonio Fico
ABSTRACT: Liquid chromatography-triple quadrupole mass spectrometry is a technique suited for rapid analysis of multiple analytes of similar and different structures and physicochemical properties. Antioxidants are a diverse group of biologically active compounds that can be present in plasma with the aim to contrast oxidative events related with atherosclerosis, arterial hypertension, stroke, myocardial infarction, diabetes, arthritis, dementia, colitis, pancreatitis, respiratory diseases, cancer, infections, etc . Current measures of antioxidant potential of plasma are based on spettrophotometry and are not able to identify the typology of antioxidants. Then, a sensitive and specific LC-MS/MS analytical method was developed for the identification and quantification of plasma antioxidants in order to assess the biological antioxidant potential of this matrix
KEY WORDS: Antioxidants, Liquid chromatography-mass spectrometry, Biological antioxidant potential
REFERENCES:
-
Halliwell B, Gutteridge JM, Cross CE (1992) Free radicals, antioxidants, and human disease: where are we now? J Lab Clin Med 119: 598-620.
-
Halliwell B, Gutteridge JMC (2015) Free Radicals in Biology and Medicine. Oxford: Oxford University Press. 944 p.
-
Cadenas E (1998) Mechanisms of Antioxidant Action. In: Özben T, editor. Free Radicals, Oxidative Stress, and Antioxidants: Pathological and Physiological Significance. Boston, MA: Springer US. pp. 237-251.
-
Kilk K, Meitern R, Harmson O, et al. (2014) Assessment of oxidative stress in serum by d-ROMs test. Free Radic Res 48: 883-889.
-
Prior RL, Gu L, Wu X, et al. (2007) Plasma antioxidant capacity changes following a meal as a measure of the ability of a food to alter in vivo antioxidant status. J Am Coll Nutr 26: 170-181.
-
Huang D, Ou B, Hampsch-Woodill M, et al. (2002) Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated beta-cyclodextrin as the solubility enhancer. J Agric Food Chem 50: 1815-1821.
-
Colombini F, Carratelli M, Alberti A (2016) Oxidative stress, d-ROMs test, and ceruloplasmin. Free Radical Research 50: 447-453.
-
Pitt JJ (2009) Principles and Applications of Liquid Chromatography-Mass Spectrometry in Clinical Biochemistry. The Clinical Biochemist Reviews 30: 19-34.
-
Want EJ, Cravatt BF, Siuzdak G (2005) The Expanding Role of Mass Spectrometry in Metabolite Profiling and Characterization. ChemBioChem 6: 1941-1951.
-
Brunnée C (1987) The ideal mass analyzer: Fact or fiction? International Journal of Mass Spectrometry and Ion Processes 76: 125-237.