Peer Reviewed Open Access Research Journal
International Journal of Medical Laboratory Research (IJMLR)
DOI: 10.35503
Research Article
Abstract
INVESTIGATION OF ANTIBIOTIC AND ANTISEPTIC RESISTANCE GENES IN METHICILLIN-RESISTANT AND METHICILLIN-SUSCEPTIBLE STAPHYLOCOCCUS AUREUS ISOLATES
Cagla Ayabaktı, Ozkan Aslantas, Mucella Bayırlı, Burcin Ozer
ABSTRACT: Aim: This study aimed to determine the antibiotic susceptibility of Staphylococcus aureus isolated from different clinical materials sent to the Microbiology Laboratory of Hatay Mustafa Kemal University Hospital, to investigate the mechanisms mediating antibiotic and antiseptic resistance, to determine the SCCmec type of methicillin-resistant isolates. Materials and Method: Overall, 187 S. aureus were included in the study. Antibiotic susceptibilities of the isolates were performed by the disc diffusion method and evaluated according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria. Antibiotic resistance, antiseptic resistance, and Staphylococcal Cassette Chromosome mec (SCCmec) types in MRSA strains were investigated using polymerase chain reaction (PCR). Results: While all of the isolates were found to be susceptible to linezolid and vancomycin; various rates of resistance for penicillin (87.1%), cefoxitin (49.93%), erythromycin (19.79%), ciprofloxacin (13.37%), tetracycline (11.23%), clindamycin (10.16%), trimethoprim-sulfamethoxazole (8.02%), gentamicin (17.82%), fusidic acid (64.2%) and rifampin (1.07%) were determined. A statistically significant difference was found between MRSA and MSSA strains in terms of MDR phenotype rates (p=0.001). Among S. aureus isolates, single resistance genes or various combinations of resistance genes were detected. SCCmec type III (52.4%) was the most common SCCmec type. Conclusions: The results of this study indicated that current control strategies should be revised to minimize antibiotic resistance and periodic surveillance studies must be carried out.
KEYWORD: Staphylococcus aureus, antibiotic resistance, antiseptic resistance, SCCmec typing
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To cite this article:
AyabaktıC, Aslantas O, Bayırlı M, Ozer B. Investıgatıon of antıbıotıc and antıseptıc resıstance genes ın methıcıllın-resıstant and methıcıllın-susceptıble Staphylococcus aureus ısolates. Int. J. Med. Lab. Res. 2022; 7,1:1-10. http://doi.org/10.35503/IJMLR.2022.71