Original Article






OJOKOH, Anthony Okhonlaye, OJO, Martha Oluwatosin



The rise in antibiotic resistance among pathogenic bacteria is a major public health concern. Hence, the need for natural safe and effective antimicrobial agents. This study therefore assayed the antibacterial efficacy of Ocimum gratissimumon multidrug resistance Klebsiella pneumoniae and Staphylococcus aureus isolated from clinical and environmental samples in Ondo State. Absolute ethanol, n-hexane and distilled water were used as extraction solvent, agar well diffusion method was used for the antibacterial efficacy of the extracts and phytochemical composition of the extracts was carried out using Analytical Methods Committee of Royal Society of Chemistry. The results showed that aqueous extract has the highest percentage yield and there was significant difference (p≤ 0.05) in quantitative phytochemical components of the extracts. N-hexane extract has the least phytochemical constituents. Among the tested K .pneumoniae and S. aureus isolated from clinical and environmental sources. S. aureus isolated from urine and K. pneumoniae isolated from wound samples were inhibited most by the extract. K. pneumoniae and S. aureus isolated from the air showed the least inhibition by the extract. Also, n-hexane extract showed the least inhibitory effect compared to the other extracts. However when extracts of the plant from different solvents were combined the inhibition surpassed that of single extract. Therefore it could be recommended that the extract of this plant be used for the treatment of infection caused by K. pneumoniae and S.aureus since the extract has demonstrated anti-Staphylococcal and anti-Klebsiella activity irrespective of the sources in-vitro.


KEY WORDS: solvent, extract, multidrug resistant, phytochemical, inhibition


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