Research Articles

      Abstract    

        

IMPROVEMENT OF “LACTULOSE-MANNITOL RECOVERY RATIO TEST” OF INTESTINAL ABSORPTION BY LC-MS\MS

 

Raffaele Conte, Andrea Cavallo, Antonio Fico

 

ABSTRACT: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has seen enormous growth in clinical laboratories during the last decades due to the fact that it offers analytical specificity superior to that of immunoassays or conventional high performance liquid chromatography. Then, most chemicals and metabolites are now assayed by LC-MS/MS. The “Lactulose\Mannitol recovery ratio (LAMA test)” is a non-invasive gastrointestinal test for the assessment of intestinal absorption and barrier function of the bowel. This article describes an optimized LC-MS/MS method used for the determination of these two sugars, characterized by rapidity, sensitivity, and easy automation. 

 

KEY WORDS: Intestinal absorption, Lactulose, Liquid chromatography-mass spectrometry, Mannitol

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