top of page
Research Articles

      Abstract    

        

HOME

TESTING THE SURFACE FIXATION METHOD IN GESTATIONAL DIABETES MELLITUS

Leicy Jazmín Ruiz Barrera, Araceli Amaya Chávez, Hugo Mendieta Zerón

 

ABSTRACT: Introduction: To test the surface fixation method contrasting urine samples of women with GDM vs healthy pregnant women. Methods: This was a pilot descriptive study. Three groups were conformed: A) Pregnant women with GDM, B) Women with healthy pregnancies and C) Non-pregnant healthy women. The positiveness of the surface fixation method was contrasted with Odds Ratio. Results: 12 women with GDM, 14 with healthy pregnancies and 9 non-pregnant women were included in the study.The OR for a positive surface fixation test when contrasting GDM vs Healthy pregnancies was of 2.7 while the value when contrasting GDM vs Healthy pregnancies + Non pregnant women was of 3.2 without reaching significant statistical difference in any case. Conclusion: the surface fixation method used with urine samples, suggests the existence of a transient antigen-antibody reaction that contributes to the inefficient insulin secretion.

 

KEY WORDS: gestational diabetes mellitus, immunodulation, surface fixation method.

REFERENCES:

  1. E, Arcidiacono B, Foti D, Brunetti A. Gestational diabetes mellitus: an updated overview. J Endocrinol Invest. 2017;40(9):899-909.

  2. Al-Ofi EA. Implications of inflammation and insulin resistance in obese pregnant women with gestational diabetes: A case study. SAGE Open Med Case Rep. 2019 Apr 22;7:2050313X19843737.

  3. Dereke J, Nilsson C, Landin-Olsson M, Hillman M. Prevalence of zinc transporter 8 antibodies in gestational diabetes mellitus. Diabet Med. 2012;29(12):e436-9.

  4. Rawal S, Tsai MY, Hinkle SN, , Zhu Y, Bao W, Lin Y, Panuganti P, Albert PS, Ma RCW, Zhang C. A Longitudinal Study of Thyroid Markers Across Pregnancy and the Risk of Gestational Diabetes. J Clin Endocrinol Metab. 2018;103(7):2447-56.

  5. Díaz Montiel JC, Gutiérrez, Fredy RS, Hinojosa Juárez AC, Ospina Alzate MI, Layton Tovar CF, Vargas Hernández JA, Mendieta Zeron H. Cytokine profile at the beginning of pregnancy in Mexican women. Cent Asian J Med Sci. 2019;5(2):78-87.

  6. Yu Z, Liu J, Zhang R,Huang X, Sun T, Wu Y, Hambly BD, Bao S. IL-37 and 38 signalling in gestational diabetes. J Reprod Immunol. 2017;124:8-14.

  7. Rudland VL, Pech C, Harding AJ, Tan K, Lee K, Molyneaux L, Yue DK, Wong J, Ross GP. Zinc transporter 8 autoantibodies: what is their clinical relevance in gestational diabetes? Diabet Med. 2015;32(3):359-66.

  8. Paun A, Danska JS. Modulation of type 1 and type 2 diabetes risk by the intestinal microbiome: Role of gut microbiome in diabetes. Pediatr Diabetes. 2016;17(7):469-77.

  9. Dudzik D, Zorawski M, Skotnicki M, Zarzycki W, Kozlowska G, Bibik-Malinowska K, Vallejo M, García A, Barbas C, Ramos MP. Metabolic fingerprint of Gestational Diabetes Mellitus. J Proteomics. 2014;103:57-71.

  10. Sugawara J, Ochi D, Yamashita R, Yamauchi T, Saigusa D, Wagata M, Obara T, Ishikuro M, Tsunemoto Y, Harada Y, Shibata T, Mimori T, Kawashima J, Katsuoka F, Igarashi-Takai T, Ogishima S, Metoki H, Hashizume H, Fuse N, Minegishi N, Koshiba S, Tanabe O, Kuriyama S, Kinoshita K, Kure S, Yaegashi N, Yamamoto M, Hiyama S, Nagasaki M. Maternity Log study: a longitudinal lifelog monitoring and multiomics analysis for the early prediction of complicated pregnancy. BMJ Open. 2019;9(2):e025939.

  11. Ruiz Castaneda M. Surface fixation; a new method of detecting certain immunological reactions. Proc Soc Exp Biol Med. 1950;73(1):46-9.

  12. Ruiz Castaneda M. Surface fixation as a practical method for diagnosis of brucellosis in man and animals. Proc Soc Exp Biol Med. 1953;83(1):36-9.

  13. Ruiz-Castaneda M. Surface fixation as a rapid test for diagnosis of toxoplasmosis. Arch Invest Med (Mex). 1977;8(1):1-4.

  14. Ruiz-Castaneda M, Naranjo Y. Rapid test for diagnosis of syphilis by means of the surface fixation method using as antigens VDRL and Treponema pallidum (strain Nichols). Arch Invest Med (Mex). 1978;9(4):559-64.

  15. Instituto de Alergias y Autoinmunidad «Dr. Maximiliano Ruiz Castañeda» [Internet]. Available in: http://iaamrc.com/en/#home

  16. MEDCALC® easy-to-use statistical software [Internet]. Available in: https://www.medcalc.org/calc/odds_ratio.php

  17. Guo Y, Han Z, Guo L, Liu Y, Li G, Li H, Zhang J, Bai L, Wu H, Chen B. Identification of urinary biomarkers for the prediction of gestational diabetes mellitus in early second trimester of young gravidae based on iTRAQ quantitative proteomics. Endocr J. 2018;65(7):727-35.

  18. López-Hernández Y, Herrera-Van Oostdam AS, Toro-Ortiz JC, López JA, Salgado-Bustamante M, Murgu M, Torres-Torres LM. Urinary Metabolites Altered during the Third Trimester in Pregnancies Complicated by Gestational Diabetes Mellitus: Relationship with Potential Upcoming Metabolic Disorders. Int J Mol Sci. 2019;20(5).

bottom of page