International Journal of Medical Laboratory Research (IJMLR)

COMPARISON ALTERATIONS OF SOME BIOCHEMICAL PARAMETERS IN RATTUS NORVEGICUS  RATS  FOLLOWING  FLUNITRAZEPAM  ADMINISTRATION

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Tonbra EE, Remi RAR, Godwin II

 

ABSTRACT: Background: Flunitrazepam is a class of benzodiazepine and a central nervous system depressant which is a sedative-hypnotic used in the treatment of anxiety, insomnia etc. This study was aimed at assessing the alterations of some biochemical parameters in Rattus norvegicus rats following flunitrazepam administration.  Methods: Each of the seven rats in experimental group one and two was administered orally with 0.8mg/1.24kg of flunitrazepam as a single dose for one day, experimental group one (acute toxicity study) and 0.2mg/1.24kg of flunitrazepam daily for a period of four weeks, experimental group two (sub-chronic toxicity study) respectively while each of the other seven rats that were monitored as controls (control group) was not administered with flunitrazepam or any other drugs. Thereafter five milliliters blood specimen were collected into lithium heparin anticoagulated bottles and spun to obtain plasma samples that were used for the quantitative measurement of alanine aminotransferase, aspartate aminotransferase, C-reactive protein, urea and creatinine. Results: The results revealed statistically significant elevations (p<0.05) in the mean values of all the measured biochemical parameters in both experimental group one and two rats respectively as compared with that of the control group.  Conclusion: In conclusion, acute and sub-chronic administration of flunitrazepam may cause hepato-renal and inflammatory disorders in Rattus norvegicus rats. It is therefore recommended that further study on the histomorphological examination of hepato-renal organs be carried out in order to ascertain the true picture of damage on these organs in the rats.

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KEY WORDS: Alterations of biochemical parameters, Flunitrazepam administration, Rattus norvegicus.

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