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Research Article





M Gupta, D Mehrotra, L Monga, N Marwah, S Singh


ABSTRACT: Introduction: Central nervous system tumors comprise 1-2% of all tumours and constitute a heterogeneous group which shows a spectrum of morphological features. Although the mainstay of diagnosis including its grading remains conventional Hematoxylin-Eosinstained histopathology but it is not always straightforward and the pathologist faces diagnostic dilemmas due to overlap in morphological features and divergent differentiation within the same tumor. Immunohistochemistry (IHC) plays a major role in the differential diagnosis and in improving the diagnostic accuracy. The present study was done to determine the utility of IHC in the diagnosis and grading of brain tumors. Patients and Methods: Thestudy was carried out between January-September 2019. The demographic, clinical and imaging details of all the cases were noted. Brain tumors were diagnosed based on examination of H&E stained sections. IHC markers were applied in difficult cases for an accurate diagnosis. Results: Out of 40 cases, 95 %were primary and 5%were metastatic tumors. Headache was most common symptom and most common site was the anterior fossa. Astrocytoma was the most common diagnosis. Grading of tumors was done as per the universal grading system. IHC was done on problematic cases to reach the final diagnosis and grading. Discussion and Conclusions: This study was carried out to highlight the importance of IHC in identifying the tumor cell type and its origin in case of metastasis. IHC also helps in assessing the proliferation index of the tumor cells, excluding reactive processes,evaluating the tumor margins and as a prognostic indicator of different types of CNS tumors.


KEY WORDS: CNS Tumors, Immunohistochemistry, Radiology, Astrocytoma, Meningioma


  1. Yeole BB. Trends in the Brain Cancer Incidence in India. Asian Pac J Cancer Prev. Apr-Jun 2008;9,2:267-70.

  2. Coons AH, Creech HJ, Jones RN. Immunological properties of an antibody containing a fluorescent group. Proc SocExpBiol Med. 1941;47,2:20

  3. Ramos-Vara JA. Technical aspects of immunohistochemistry. Vet Pathol 2005;42,4;405-26.

  4. Madabhushi V, Venkata RI, Garikaparthi S, Kakarala SV, Duttaluru SS. Role of immunohistochemistry in diagnosis of brain tumors: A single institutional experience. J NTR Univ Health Sci. 2015; 4,2: 103-11.

  5. Mondal S, Pradhan R, Pal S, Biswas B, Banerjee A, Bhattacharyaya D. Clinicopathological pattern of brain tumors: A 3-year study in a tertiary care hospital in India. Clin Cancer Investig J. 2016; 5,5: 437-40.

  6. Bhati S, Mittal A, Mehta J.Role of Immunohistochemistry in diagnosis and grading of brain tumours. JMSCR. 2018;6,3:1138-46.

  7. Desai RI, Soni NS, Desai IM, Rajput K. Retrospective study of CNS tumors in tertiary care Hospital. Int J Cur Res Rev.2017;9,2:1-4.

  8. Andrews NB, Ramesh R, Odjidja T. A preliminary survey of central nervous system tumors in Tema, Ghana. West Afr J Med. 2003; 22,2:167-72.

  9. Torres LF, Almeida R, Avila S, Alessi S, Freitas R. Brain tumours in South Brazil: a retrospective study of 438 cases. ArqNeuropsiquiatr. 1990 ; 48,3: 279-85.

  10. Anadure HN, Ravindra RS, Karnappa AS. Morphological patterns of intracranial; lesions in a tertiary care hospital in north Karnataka: A cliicopathological and immunohistochemical study. Journal of Clinical and Diagnostic Research.2016 ;10,8:EC01-EC05.

  11. Aryal G. Histopathological pattern of central nervous system tumor: A three-year retrospective study. Journal of Pathology of Nepal. 2011;1:22-25.

  12. Pradhan R, Mondal S, Pal S, Chatterjee S, Bandyapadhyay A, Bhattacharyay D. Clinicopathological study of brain tumors with special reference to atypical cases: A retrospective analysis of 130 cases in a tertiary care hospital of India. Int J Med Res Prof. 2017;3,3:143-48.

  13. Ghanghoria S, Mehar R, Kulkarni CV, Mittal M, Yadav A, Patidar H. Retrospective histological analysis of CNS tumours – A 5-year study. Int J Med Sci Public Health. 2014;3,10:1205-7.

 To cite this article:

Gupta M, Mehrotra D, Monga L, Marwah N, Singh S. Study of the morphological patterns of intracranial lesions in a tertiary care hospital in haryana: a clinicopathological and immunohistochemical study. Int. J. Med. Lab. Res. 2021; 6,2:7-13.

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