International Journal of Medical Laboratory Research (IJMLR)

SPECTROSCOPICAL METHOD FOR ESTIMATION OF ATENOLOL AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICAL DOSAGE FORM

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Sai Datri A, Lakshmana Rao A, Chandini U, Anil Kumar V, Prudhvi Raj V, Veera Naga Anjali V

 

ABSTRACT: Introduction: A sensitive and validated spectroscopic estimation of Atenolol and Hydrochlorothiazide in pharmaceutical dosage form, without prior separation, by three different techniques (Simultaneous Equation, Dual Wavelength Method, and Derivative Spectroscopic Method) has been developed.  Method: The works were carried out on Shimadzu electron UV1800 double beam UV-Visible spectrophotometer. The absorption spectra of reference and test solutions were carried out in 1 cm matched quartz cell over the range of 200 - 400 nm. The linearity ranges for Atenolol and Hydrochlorothiazide were 2-10 μg/ml and 1-5 μg/ml. Conclusion: The results of the analysis have been validated statistically and by recovery studies. The proposed procedures are rapid, simple, require no preliminary separation steps, and can be used for routine analysis of both drugs in quality control laboratories.

 

KEY WORDS: Atenolol, Hydrochlorothiazide, UV spectroscopy, and Validation.

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