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IMMUNOSTIMULANT AND FREE RADICAL SCAVENGING STUDIES OF GANODERMA APPLANATUM

 

Madhu. Divakar*, Lins Mary Joy

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Abstract: Objective: The present animal experimentation study is planned to screen the immunostimulant efficacy, free radical scavenging potential and the acute toxicity of the bracket fungus, Ganoderma applanatum . Methods: The study analyse the invitro immunostimulant activity by phagocytic index determination using Candida albicans, acute toxicity study by brine shrimp assay and the free radical scavenging activities of G. applanatum solvent extracts. Conclusion: The percentage immunostimulation was found to be 88% for the chloroform-methanol (1:1) Ganoderma applanatum extract (GAE). The free radical scavenging activity studies were performed by utilising invitro model of hydroxyl, superoxide and lipid peroxide radical generating system. Results indicated that GAE at 100 mcg/ml showed 88.1, 89.19, and 81.87 % scavenging activity of hydroxyl, superoxide and lipid peroxide radical respectively. The LC50 was determined using brine shrimp assay method and calculated as 875 mcg /ml. The present study shows that G. applanatum can be utilised as an effective natural supplement to impart immunostimulation. 

 

KEYWORDS: Ganoderma applanatum, Immunostimulant activity, free radical scavenging activity 

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REFERENCES:​

  1.  Vijay H.M., Comtois P., Sharma R., Lemieux R., Allergenic components of Ganoderma applanatum, Grana.1991, 30:1, 167-170.

  2. Ginns, James (2017). Polypores of British Columbia (Fungi: Basidiomycota). Victoria, BC: Province of British Columbia - Forests, Lands, and NR Operations. p. 105. ISBN 978-0-7726-7053-3.

  3. Goldman, Gary B. (2019). Field guide to mushrooms & other fungi in South Africa. Marieka Gryzenhout. Cape Town, South Africa.  ISBN 978-1-77584-654-3. OCLC 1117322804

  4. IL Finar, Stereochemistry and Chemistry of Natural products, Volume 2, 5th edn, 158, 1975.

  5. Boldizsar I, Horvath K, Szedlay K.G, Molnar-Perl, Simultaneous GC-MS quantitation of acids and sugars in the hydrolyzates of immunostimulant, water-soluble polysaccharides of basidiomycetes, Chromatographia. 47(7-8) (1998) 413-419.

  6. Elkhateeb, Waill A.; Zaghlol, Gihan M.; El-Garawani, Islam M.; Ahmed, Eman F.; Rateb, Mostafa E.; Abdel Moneim, Ahmed E. (2018-05-01). "Ganoderma applanatum secondary metabolites induced apoptosis through different pathways: In vivo and in vitro anticancer studies". Biomedicine & Pharmacotherapy. 101: 264–277.  doi: 10.1016/j.biopha.2018.02.058

  7. Moradali, Mohammad-Fata; Mostafavi, Hossien; Hejaroude, Ghorban-Ali; Tehrani, Abbas Sharifi; Abbasi, Mehrdad; Ghods, Shirin (2006). "Investigation of Potential Antibacterial Properties of Methanol Extracts from Fungus Ganoderma applanatum". Chemotherapy. 52 (5):241–244. doi:10.1159/000094866

  8. Mohammad Shahadat Hossain, Anik Barua, Mohammad Akramul Hoque Tanim, Mohammad Sharif Hasan, Mohammad Jahedul Islam, Md. Rabiul Hossain, Nazim Uddin Emon, and S M Moazzem Hossen. Ganoderma applanatum mushroom provides new insights into the management of diabetes mellitus, hyperlipidemia, and hepatic degeneration: A comprehensive analysis. Food Sci Nutr. 2021 Aug; 9(8): 4364–4374.

  9. Kulkarni SR, Karnade VS, Study of immunopotentiating activity of naphthoquinione extracts of leaves of Lawsonia alba, 1983, Indian drugs, 35(7), 427-433.

  10. Mayer B.N., Ferrigni. N.R., Putname J.E., Jacobson L.B., Nichols D.E., and McLaughlin J.L., Brine shrimp: A convenient general bioassay for active plant constituents, Planta Med., 1982, vol 45, 31-34.

  11. Halliwell B., Gutteridge J. M. C. and Aruoma O. I., The deoxyribose method: A simple test tube assay for determination of rate constants for reactions of hydroxyl radicals., Analytical Biochemistry, 1987, 165, 215- 219.

  12. Savitha C, Thripathi P, 1995, Indian Journal of Experimental Biology, 33,428.

  13. Rajshree CR, Rajmohan T, Augusti KT. Antiperoxide effect of garlic protein in alcohol fed rats. Indian Journal of Experimental Biology. 1998 Jan; 36 (1):60-64.

  14. Keith Hyland, Emmanuelle Voisin, Hélène Banoun, Christian Auclair, 1983, Aanalytical Biochemistry, 135, 280-287.

 To cite this article:

CD Madhu, JM Lins. Immunostimulant and free radical scavenging studies of ganoderma applanatum . Int. J. Med. Lab. Res. 2023; 8,1:21-25. http://doi.org/10.35503/IJMLR.2023.8103

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