International Journal of Medical Laboratory Research (IJMLR)

TOTAL PROTEIN, ALBUMIN AND SERUM PROTEIN ELECTROPHORESIS PATTERN OF ALCOHOLICS IN IKERE-EKITI, EKITI STATE

 

OMON AE, AJAYI OD, EDET OO, OSHOBUGIE BN, ALAKE OA

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Abstract: Plasma proteins such as albumin are indispensible in maintaining numerous essential roles of living cells. This objective of this study was to determine the total serum protein, albumin and serum protein electrophoresis pattern of alcoholics. One hundred samples comprising sixty (60) subjects (alcohol users) and forty controls (non-alcohol users) were recruited for this study. Total protein and albumin were determined using spectrophotometric methods and protein electrophoresis respectively. The results were presented in tables and chat as mean ± standard deviation. Statistical analysis was done using one way analysis of variance (ANOVA) and Student’s t-test using IBM SPSS version 24.0. A p-values of <0.05 was considered significant. The results obtained showed that the mean value of total protein in subjects and control was 50.22±8.44 g/L and 74.38±8.12 g/L, albumin was 30.89±5.05 g/L and 45.13±5.18 g/L, globulin was 19.33±6.11 g/L and 29.25±5.36 g/L, while albumin/globulin ratio was 1.6 and 1.54 respectively. Statistically, total protein, albumin and globulin were significantly lower (p<0.05) in subjects compared with control group. This study concludes that alcohol consumption significantly reduced the level of total protein, albumin and globulins. The combination of these parameters in combinations may be a useful indicator for identification and determination of severity of alcoholic liver diseases. Regular screening exercise of alcoholics should be carried out to detect individuals at risk of hepatic dysfunction.

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KEYWORDS: Alcoholics, Plasma proteins, Protein Electrophoresis, Total protein, Albumin

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REFERENCES:​

1. [1] Pohl K, Moodley P, Dhanda AD. Alcohol’s Impact on the Gut and Liver. Nutrients 2021; 13,9: 3170.

2. [2] Manary MJ, Trehan I. Protein-energy malnutrition. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine. 25th ed. Philadelphia, PA: Elsevier Saunders; 2016; Pp. 215.

3. [3] Landry DW, Bazari H. Approach to the patient with renal disease. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine. 25th ed. Philadelphia, PA: Elsevier Saunders, 2016; Pp. 114-118.

4. [4] Belinskaia DA, Voronina PA, Shmurak VI, Jenkins RO, Goncharov NV. Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties. International Journal of Molecular Sciences 2021; 22,19: 10318.

5. [5] Dispenzieri A, Gertz MA, Therneau TM, Kyle RA. Retrospective cohort study of 148 patients with polyclonal gammopathy. Mayo Clinic Proclation 2016; 76 ,6, 476–487.

6. [6] George ED, Sadovsky R. Multiple myeloma: recognition and management. American Family Physician 2014; 59 ,9: 1885–1894.

7. [7] Ramanathan S, Narasimhachar SC. Serum Protein Electrophoresis and Its Clinical Applications. IntechOpen. 2020.

8. [8] Raneem O. Effects of alcohol on haemostasis. American Journal of Clinical Pathology 2018; 123 ,1: 96-105.

9. [9] Sawadogo-Lingani H, Owusu-Kwarteng J, Glover R, Diawara B, Jakobsen M, Jespersen L. Sustainable Production of African Traditional Beers With Focus on Dolo, a West African Sorghum-Based Alcoholic Beverage. Frontiers in Sustainable Food Systems 2021; 5: 672410.

10. [10] Matsushita H, Takaki A. Alcohol and hepatocellular carcinoma. BMJ Open Gastroenterol 2019; 6,1: 260-266. 

11. [11] Rachdaoui N, Sarkar DK. Pathophysiology of the Effects of Alcohol Abuse on the Endocrine System. Alcohol Research 2017; 38,2: 255-276.

12. [12] Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative Stress: Harms and Benefits for Human Health. Oxidative Medicine & Cell Longevity 2017; 17: 841-846.

13. [13] Kamran U, Towey J, Khanna A, Chauhan A, Rajoriya N, Holt A. Nutrition in alcohol-related liver disease: Physiopathology and management. World Journal of Gastroenterology 2020; 26,22: 2916-2930.

14. [14] Dixon JB, Dixon ME, O’Brien PE. Reduced plasma homocysteine in obese red wine consumers: A potential contributor to reduced cardiovascular risk status. European Journal of Clinical Nutrition, 2015; 56: 608-614.

15. [15] Burger M, Mensink A, Bronstrup W, Thierfelder G, Pietrzik K. Alcohol consumption and its relation to cardiovascular risk factors in Germany. European Journal of Clinical Nutrition 2018; 58: 605-614.

16. [16] Duze D. Ekiti: the land of genius. Hope and Faith Press, Ado-Ekiti, 2005; Pp. 44-46.

17. [17] Soeters PB, Wolfe RR, Shenkin A. Hypoalbuminemia: Pathogenesis and Clinical Significance. Journal of Parenteral & Enteral Nutrition 2019; 43,2: 181-193.

18. [18] Igwe CU, Ewuga EE, Ujowundu CO, Onyeocha IO, Onwuliri VA. Serum protein concentration and amino acid profile of HIV/HBV co-infected subjects on HAART in Plateau State, Nigeria. African Health Sciences 2022; 22,1: 418-430.

19. [19] Hashash JG, Koutroumpakis F, Anderson AM, Rivers CR, Hosni M, Koutroubakis IE, Ahsan M, Gkiaouraki E, Dunn MA, Schwartz M, Barrie A, Babichenko D, Tang G, Binion DG. Elevated serum globulin fraction as a biomarker of multiyear disease severity in inflammatory bowel disease. Annals of Gastroenterology 2022; 35,6: 609-617.

20. [20] Keller U. Nutritional Laboratory Markers in Malnutrition. Journal of Clinical Medicine 2019; 8,6: 775.

21. [21] Jang ES, Jeong SH, Hwang SH. Effects of coffee, smoking, and alcohol on liver function tests: a comprehensive cross-sectional study. British Medical Council of Gastroenterology 2012; 12: 145-148

22. [22] Lakshimi B, Abhilekh D, Jishan P. Serum Protein and Its Fractions in Patients of Alcohol Dependence and Healthy Individuals – A Comparative Study. Journal of Medical Science and Clinical Research 2017; 5 (10): 29596-29603

23. [23] Torruellas C, French SW, Medici V. Diagnosis of alcoholic liver disease. World Journal of Gastroenterology 2014; 20 ,33: 11684-11699.

24. [24] Queremel M, Jialal I. Urinalysis. In: StatPearls. Treasure Island (FL): StatPearls Publishing. 2022. Available from: https://www.ncbi.nlm.nih.gov /books/NBK557685/

25. [25] Flores-Bonilla A, Richardson HN. Sex Differences in the Neurobiology of Alcohol Use Disorder. Alcohol Research 2020; 40,2: 04-08

26. [26] Joven J, Cliville X, Camps J, Espinel E, Simo J, Vilella E, Oliver A. Plasma protein abnormalities in nephrotic syndrome: effect on plasma colloid osmotic pressure and viscosity. Clinical Chemistry 2017; 4,3: 1223–1231

27. [27] Osna NA, Donohue TM Jr, Kharbanda KK. Alcoholic Liver Disease: Pathogenesis and Current Management. Alcohol Research 2017; 38,2: 147-161.

28. [28] Ohwada H, Nakayama H, Kanaya Y, Tanaka Y. Serum albumin levels and their correlates among individuals with motor disorders at five institutions in Japan. Nutritional Research Practice 2017; 11,1, 57-63.

29. [29] So E, Joung H. Alcohol Consumption Reduces the Beneficial Influence of Protein Intake on Muscle Mass in Middle-Aged Korean Adults: A 12-Year Community-Based Prospective Cohort Study. Nutrients 2019; 11,9: 2143.

30. [30] Juma A, Njagi EN, Ngeranwa JJ. Estimation of reference values for liver function tests for adult population in North Rift Valley, Kenya. Asian Journal of Medical Sciences 2011; 3,6: 243-249.

31. [31] van de Wouw J, Joles JA. Albumin is an interface between blood plasma and cell membrane, and not just a sponge. Clinical Kidney Journal 2021; 15,4: 624-634.

32. [32] Foster BM, Abdollahi A, Henderson GC. Alterations in the Plasma Protein Expression Pattern in Congenital An albuminemia—A Systematic Review. Biomolecules 2023; 13,3: 407.

33. [33] Priya N, Venkatalakshmi P. The Impact of heavy alcohol consumption and cigarette smoking on liver function – A Clinical Survey. International Journal of Pharmacy and Pharmaceutical Sciences 2013; 5 ,4: 82-85.

34. [34] Das SK, Vasudevan DM. Biochemical Diagnosis of Alcoholism. Indian Journal of Clinical Biochemistry 2005; 20 ,1: 35-42

35. [35] Pratik K, Jose A, Rodriguez AR. Alcoholic Hepatitis: A Common Disease with Uncommon Presentation. Case Report in Gastroenterology 2020; 14 (2): 448–452.

36. [36] Wang H, Gao P, Chen W. A cross-sectional study of alcohol consumption and alcoholic liver disease in Beijing: based on 74,998 community residents. British Medical Council of Public Health 2022; 22:723

37. [37] Gori E, Pierini A, Tulone F, Abramo F, Marchetti V. Serum protein electrophoresis in 26 dogs with chronic hepatitis. Journal of Veterinary Diagnosis & Investigations 2022; 34,4: 738-741.

38. [38] Marshall T, Vesterberg O, Williams KM. Effects of alcohol abuse on human serum proteins revealed by two-dimensional electrophoresis. Electrophoresis 2014; 5 ,1, 122-128.

39. [39] Vavricka S, Burri E, Beglinger C, Degen L, Manz M. Serum Protein Electrophoresis: An Underused but Very Useful Test. Digestion 2019; 79 ,9: 203-210.

40. [40] Sharma A, Nagalli S. Chronic Liver Disease. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023. Available from: https://www.ncbi.nlm.nih.gov/books/NBK554597/