International Journal of Medical Laboratory Research (IJMLR)

ROUTINE HISTOPATHOLOGY TECHNIQUES: A COMPREHENSIVE REVIEW OF LABORATORY PRACTICES AND RECENT ADVANCEMENTS

 

Syed Mazher Ali Ibrahim Ali, Sayyed Sana Anjum

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ABSTRACT:

Histopathology is a cornerstone of diagnostic medicine, providing microscopic insight into tissue pathology and guiding clinical decisions. Routine techniques—including tissue fixation, processing, embedding, sectioning, staining, and mounting—are fundamental for producing high-quality slides for microscopic evaluation. This review comprehensively summarizes each step of the routine histopathological workflow, focusing on standard practices, commonly used reagents, and procedural challenges. Additionally, we highlight recent advancements, such as automation and microwave-assisted techniques, which improve turnaround time and diagnostic accuracy. Understanding these foundational laboratory methods is essential not only for maintaining quality and reliability but also for integrating newer technologies in diagnostic pathology.

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REFERENCES:​​

1. Bancroft JD, Gamble M. Theory and Practice of Histological Techniques. 7th ed. Churchill Livingstone; 2013.

2. Kiernan JA. Histological and Histochemical Methods: Theory and Practice. 4th ed. Scion Publishing; 2008.

3. Culling CFA. Handbook of Histopathological and Histochemical Techniques. 3rd ed. Butterworths; 1974.

4. Suvarna SK, Layton C, Bancroft JD. Bancroft’s Theory and Practice of Histological Techniques. 9th ed. Elsevier; 2023.

5. Kumar V, Abbas AK, Aster JC. Robbins and Cotran Pathologic Basis of Disease. 10th ed. Elsevier; 2021.

6. Prophet EB, Mills B, Arrington JB, Sobin LH. Laboratory Methods in Histotechnology. Washington DC: AFIP; 1992.

7. Carson FL, Hladik C. Histotechnology: A Self-Instructional Text. 3rd ed. Chicago: ASCP Press; 2009.

8. Dey P. Basic and Advanced Laboratory Techniques in Histopathology and Cytology. Springer; 2018.

9. Avwioro OG. Histochemical uses of hematoxylin – A review. JPCS. 2011;1(1):24–34.

10. Ross MH, Pawlina W. Histology: A Text and Atlas. 7th ed. Wolters Kluwer; 2015.

11. Kumar GL, Kiernan JA. Education Guide: Special Stains and H&E. Dako; 2010.

12. Horobin RW, Kiernan JA. Conn's Biological Stains. 10th ed. BIOS Scientific; 2002.

13. Luna LG. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd ed. McGraw-Hill; 1968.

14. Cook HC. Manual of Histological Demonstration Techniques. 2nd ed. Butterworths; 1996.

15. Titford M. Progress in the development of microscopical techniques for diagnostic pathology. J Histotechnol. 2009;32(1):9–19.

16. Buesa RJ. Histology without xylene. Ann Diagn Pathol. 2007;11(2):109–16.

17. Chiu KY, Ying L, Ng TP, Yau WP. Evaluation of microwave tissue processing. Histopathology. 2001;39(3):261–7.

18. Leong AS-Y. Principles and Practice of Surgical Pathology. Springer; 2004.

19. Wang Z, Zheng S, Zheng D. Digital pathology: Current status and future perspectives. Front Med. 2020;14(1):1–10.

20. Pantanowitz L, Sinard JH, Henricks WH, Fatheree LA, Carter AB, Contis L, et al. Whole slide imaging and digital pathology. J Pathol Inform. 2011; 2:36.

21. Kim JY. Digital pathology and artificial intelligence in pathology. Pathol Res Pract. 2020;216(9):153040.

22. Ramos-Vara JA. Technical aspects of immunohistochemistry. Vet Pathol. 2005;42(4):405–26.

23. Miettinen M. Immunohistochemistry of soft tissue tumours. Histopathology. 2005;46(1):87–93.

24. Weiss LM, Chu P, Shintaku IP. Immunohistochemistry in hematopathology. Cancer Treat Res. 2005; 127:119–47.

25. Gatter KC. Techniques in Immunocytochemistry. Springer; 1992.

26. De Bournonville S, Toubeau G, Behets C, Gillard J, Joris J, Meurisse M, et al. Contrast-enhanced micro-CT for virtual 3D anatomical pathology of biological tissues: polyoxometalates for simultaneous imaging of soft and mineralized skeletal tissues. 26th European Congress on Obesity. Glasgow, Scotland; 2019.

27. Dorward DA, Russell CD, Um IH, Elshani M, Armstrong SD, Penrice-Randal R, et al. Tissue-specific immunopathology in fatal COVID-19. Am J Respir Crit Care Med. 2021;203(2):192–201. Available from:

28. Wick MR. The hematoxylin and eosin stain in anatomic pathology—An often-neglected focus of quality assurance in the laboratory. Semin Diagn Pathol. 2019;36(5):303–11.

29. Magaki S, Ganesh T, Chang SY, Kim R. An introduction to the performance of immunohistochemistry. In: Biobanking: Methods and Protocols. New York: Springer; 2019. p. 289–98.