International Journal of Medical Laboratory Research (IJMLR)

HISTOMORPHOMETRIC CHANGES IN SHEEP GUT FOLLOWING NUTRITIONAL SUPPLEMENTATION WITH SACCHAROMYCES CEREVISIAE FERMENTATION PRODUCT (DIAMOND V XPC™)

 

M Wanjohi, J Gikunju, A N. Makanya, D N. Karanja, C K. Gachuiri, CJ Ng'ang'a 

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ABSTRACT:

Saccharomyces cerevisiae fermentation product (Diamond V XPC™) was evaluated for effects on abomasal and small intestinal histomorphometry in Dorper lambs using qualitative and quantitative methods. Ninety lambs were weighed from birth at two-week intervals to six weeks, stratified by body weight, and assigned to two groups: control group (C1) and a treatment group (E2) receiving 2 g/head/day of the fermentation product through one-month post-weaning. Growth was monitored and at experiment end five lambs per group were randomly selected. They were euthanized, abomasa and small intestines excised, washed, fixed, and processed for stereological and histological analysis with Stepanizer software. Histomorphometric results indicated improved mucosal organization and more prominent intestinal villi in sheep supplemented with Saccharomyces cerevisiae fermentation product compared with the control group. Mean abomasal volumes were 116.01 ± 15.40 cm³ (C1) and 148 ± 87.71 cm³ (E1) intestinal volumes were 610.15 ± 99.9 cm³ and 564 ± 81.4 cm³, respectively. Villous length increased significantly with supplementation (503 ± 5.20 µm in C1 vs. 537.67 ± 37.98 µm in E1). Intestinal mucosal surface area rose from 4,641.63 ± 261.89 cm² to 7,038.59 ± 1,972.56 cm² with treatment; abomasal mucosal surface area increased from 200 ± 14.4 cm² to 380.67 ± 98.14 cm². Overall, S. cerevisiae supplementation produced statistically significant mucosal adaptations indicative of enhanced digestive and absorptive capacity in the gut. The study provides detailed histological evidence addressing an existing knowledge gap and supports the physiological relevance of S. cerevisiae fermentation products for improving gut health and informing feeding strategies in sheep.

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Keywords: Saccharomyces cerevisiae fermentation product, sheep gut, stereology, histomorphometry

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