top of page
Research Articles






Renjith Chandran, Srinivasa Kannan S.R, Thanga Mariappan  K, Shabeena Merrin J

ABSTRACT: Antimicrobial resistance pattern of Bloodstream infections in patients with suspected bacteremia from Kanyakumari District, Tamil Nadu, India was surveyed for a period of 6 months and cultured as per the methods employed by CLSI. Antibiotic sensitivity was tested using Kirby-Bauer disc diffusion method.  A total of 295 bacteremia suspected patient’s blood culture samples were processed, of which 6 bacterial pathogens isolated from 27 positive blood cultures, among which 67% were gram-positive and 33% were gram-negative.The predominant isolate was coagulation negative Staphylococcusspp. (CoNS) (37%). The other isolates were Staphylococcus aureus (30%), Escherichia coli (18%), Pseudomonas aeruginosa (7%), Klebsiella pneumoniae (4%), and Enterococcus spp. (4%). The pathogens coagulase negative Staphylococcus spp and Staphylococcus aureus were more commonly resistant to Co-trimoxazole (50 to 68%) and Penicillin G (83 to 90%). Bloodstream infections are important causes of morbidity in patients, especially among the age group of 1-20 years. Prescription of proven resistant antibiotics to suspected bacteremic patients’ needs utmost attention in the study region.

KEYWORDS: Bloodstream infection- Bacterial Pathogens - Antibiotic resistance and sensitivity



  1. Castagnola E., Caviglia I., Pistorio A., Fioredda F., Micalizzi C., Viscoli C. Blood stream infections and invasive mycoses in children undergoing acute leukemia treatment. Eur.J.Cancer. 2005; 41(10): 1439-445.

  2. Chaudhry I., Chaudhry NA., Munir M., Hussain R., Tayyab M. Etiological pattern of septicemia at three hospitals in Lahore. JCPSP.Biomedica.2009; 25(8): 101-105.

  3. Cockeril F R., Wilson J W., Vetter E.A. Optimal testing parameters for blood cultures. Clin.Infect.Dis. 2004; 38: 1724-30.

  4. Ern Gutschik. Microbiological recommendations for the diagnosis and follow-up of infective endocarditis. Clin.Microbiol.Infect 1998; 4: 3S10-3S16.

  5. Freedman S.B., Roosevelt G.E. Utility of anaerobic blood cultures in a pediatric emergency department. Pediatr.Emerg.Care. 2004;20(7):433-6

  6. Garey K.W., Rege M., Manjunath P.,Pai, Mingo D.E., Suda K.J., Turpin R.S., Bearden D.T. Time to initiation of fluconazole therapy impacts mortality in patients with Candidemia: A Multi-Institutional Study. Clin.Infect.Dis. 2006;43(1):25-31

  7. Gray JW. A 7-year study of bloodstream infections in an English children’s hospital. Eur.J.Pediatr 2004; 163(9):530-535.

  8. Kalantar E., Motlagh M., Lordnejad H., Beiranvand S.The prevalence of bacteria isolated from blood cultures of Iranian children and study of their antimicrobial susceptibilities in Jundishapur. Journal of Natural Pharmaceutical Products. 2008; 3(1): 1-7.

  9. Lincoln S M., Carlos Euardo M B., Luiza Z M., Paulo Cezar C A., David S G.,  Rolf G., Marcus Castro F. Bloodstream infections by multidrug-resistant bacteria in patients in an intensive care unit for the treatment of burns: a 4-year-experience. Rev.Bras.Cir. Plást. 2012; 27(3):374-8

  10. Michael A., Pfaller, Ronald N., Jones, Gary V., Doern, Kari Kugler. Bacterial pathogens isolated from patients with bloodstream infection: frequencies of occurrence and antimicrobial susceptibility patterns from the SENTRY. Antimicrobial Surveillance Program (United States and Canada. Antimicrobial Agents and Chemotherapy. 1998; 1762–1770.

  11. Mitta P., Adamson K., Loivukene K.,, Lang C., Telling A. , Parod , K.,  Roome A.,  Naaber B. , Maimets M. Epidemiology of nosocomial bloodstream infections in Estonia. Journal of Hospital Infection. 2009; 71: 365 - 370

  12. Monica Cheesbrough. District laboratory practice in tropical countries. Cambridge University Press, Newyark. 2004:2: 1-4.

  13. Mucheye G., Alemayehu G., Tigist A., Tinebeb T., Agersew A., Biniam M. Bacterial profile and antimicrobial susceptibility pattern in septicemia suspected patients attending Gondar University Hospital, Northwest Ethiopia.BMC Research Notes. 2013; 6:283.

  14. Opota O., Croxatto A., Prod’hom, G., Greub G.Blood culture-based diagnosis of bacteraemia: state of the art. Clinical Microbiology and Infection. 2015; 21(4):314 -322.

  15. Prakash K P., Vinod A., Geethanjali P P.  Bloodstream bacterial pathogens and their antibiotic resistance pattern in Dhahira Region, Oman. Oman Medical Journal. 2011; 26(4): 240-247.

  16. Reynolds R., Potz N., Colman M., Williams A., Livermore D., MacGowan A. BSAC extended working party on bacteraemiaresistance surveillance. Antimicrobial susceptibility of the pathogens of bacteraemia in the UK and Ireland 2001-2002: the BSAC Bacteraemia Resistance Surveillance Programme. J AntimicrobChemother. 2004; 53(6):1018-1032.

  17. Sader HS., Gales AC., Pfaller MA., Mendes RE., Zoccoli C., Barth A. Pathogen frequency and resistance patterns in Brazilian hospitals: summary of results from three years of the SENTRY antimicrobial surveillance program. Braz J Infect Dis. 2001;5(4):200-214.

  18. Vijaya Devi A., Biswajeet S., Damrolien S., Praveen S H., Phangreichon L., Ksh.Mamta D. A Study on the Bacterial Profile of Bloodstream Infections in Rims Hospital. Journal of Dental and Medical Sciences. 2015; 14(1): 18-23

bottom of page