International Journal of Medical Laboratory Research (IJMLR)

QUALITATIVE AND QUANTITATIVE ANALYSIS OF BILIARY CALCULI IN A TERTIARY CARE HOSPITAL

 

J Kaur, G Goyal, S Singh

 

ABSTRACT:Background :- Gallstone disease remains a serious health concern for human beings, affecting millions of people throughout the world. Material and method:- The cross sectional study was conducted in department of Biochemistry and Surgery in Guru Gobind Singh Medical College and Hospital, Faridkot. 100 patients of chollithiasis were enrolled for study. Physical and chemical analysis of stones was done. Result:- Out of 100 gallstone patients, 36 were male and 64 were females. The biliary calculi divided into three categories, these are cholesterol stone(33), pigment stone (25)and mixed stones(42). The qualitative and quantitative analysis of biliary calculi showing the maximum amount of cholesterol was present in cholesterol stones(452.24 ± 37.02mg/gm). The maximum amount of bilirubin was present in pigment stones (4.26 ± 0.42mg/gm).The maximum amount of calcium was present in pigment stones(19.28 ± 2.10mg/gm). Mixed type of stone is more common than the cholesterol and pigmented stones. Conclusion:- From the present study, we concluded that the mean age of the patients with gall stones was 48.19 ± 10.72  years with a male-female ratio of 1:1.7. Mixed type of stone is more common than the cholesterol and pigmented stones. The categorization of gall stones based on chemical composition is required for understanding path physiology of gall stones. These manual methods are easy and cheap to do quantitative analysis of gall stones.

 

KEYWORDS: gall stones, cholelithiasis

​

REFERENCES:

1. J.W. . 1st ed. Boca Raton: CRC Press.1994: 4.

2. Guyton CA, Hall E J. .11th ed. Philadelphia: W.B. Saunders.2005: 802–04.

3. Jayanthi V, Palanivelu C, Prasanthi R, Methew S, Srinivasan V. Composition of gallstones in Coimbatore district of Tamil Nadu State. Ind J Gastroenterol. 1998;17:134-35.

4. Malhotra SL. Epidemiological study of cholelithiasis among railroad workers in India with special reference to causation. Gut.1968; 9(3): 290-95.

5. Tandon RK. Pathogenesis of gallstones in India.Trop Gastroenterol. 1988; 9: 83-91.

6. Internal Clinical Guidelines Team (UK). Gallstone Disease: Diagnosis and Management of Cholelithiasis, Cholecystitis and Choledocholithiasis. National institute for health and care excellence:Clinical Guideline.2014.

7. AL Atabi M, Chin BS, Luo YX.Experimental investigation of the flow of bile in patient specific cystic duct models. Journal of biomechanical engineering. 2010.

8. Fransisca P, Dyah NI. Gallstone Analysis. The Indonesian journal of gastroenterology, Hepatology and Digestive Endoscopy. 2016; 17:124-30.

9. Longstreth FG, Zieve D. Gallstones—Cholelithiasis, Gallbladder attack, Biliary colic, Gallstone attack, Bile calculus, Biliary calculus. Obstetrix medical group.2009.

10. Channa AN, Khand DF, Khand UT, Leghari HM, Memon NA. Analysis of human gallstones by Fourier Transform Infrared (FTIR). Pakistan Journal of Medical Sciences.2007; 23 (4): 546–50.

11. Trinder,p. Ann. Clin biochem.1969; 6:24.

12. Allain CC . Ann. Clin biochem.1974; 20:470.

13. Flegg HM. Ann. Clin biochem.1972; 10:79.

14. Malloy HT and Evelyn KA. J. Bio chem.1937;119:481.

15. Baginski ES, Marie SS, Clarke WL, Zak. Clin chemistry. Acta 1973;46:49.

16. Gomorri GJ. Lab clin Chem. Acta 1973; 46:49.

17. Chandran P, Kuchhal NK, Garg P, Pundir CS. An extended chemical analysis of gallstone. Indian Journal of Clinical Biochemistry.2007;22 (2): 145-150.

18. Das B, Malik KA, Rehman A, Samanta S, Ahsan M. Quantitative Analysis of Chemical Composition of Gallstones in North Indian Population (Rohilkhand Region, Uttar Pradesh). NJIRM .2014; 5(4): 4-12.