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THE EFFECT OF INTERMITTENT NEGATIVE PRESSURE ON COLLAGEN CONCENTRATION IN MICE SKIN

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OS Abbadi , AH Osman , NAM Ali 

 

ABSTRACT: Overview: Physical forces are known to affect biological structure and modulate the extracellular matrix. Collagen is the most abundant protein outside the cell, and it is clearly highlighted through Masson trichrome and van Geison’s stain. This research aimed to calculate the specific concentration of collagen within histology slides, after being subjected to sessions of intermittent negative pressure (INP), using Adobe® Photoshop® software and mice models. Objectives: To measure the collagen concentration in histology slides through Adobe® Photoshop®, using two types of stains: Masson trichrome, and van Gieson’s stain. Methods: Sixteen mice were put into sessions of INP ten minutes daily, for three weeks. Skin tissues were extracted from test and control sides. Stained slides were photographed and inserted in the Adobe® Photoshop® program to measure collagen concentration according to the specified calculated areas within the slides. Results: Masson’s trichrome slides gave a mean Collagen concentration of (58.4%) in the test slides, compared to the control which was (39.9%). In van Gieson stained slides, the collagen gave a mean concentration of (54.93%) in test slides and (41.32%) in the control. The difference was statistically insignificant in both staining.  Conclusion: Applying INP to mice skin for three weeks did not significantly change the concentration of the collagen in the extracellular matrix

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KEY WORDS: Collagen, Negative pressure, Masson’s trichrome, van Gieson stain.

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REFERENCES:

  1. Richard-Blum S. The collagen family. Cold Spring Harb. Perspect Biol. Jan. 2011; 3(1): a004978. DOI: 10.1101/cshperspect.a004978.

  2. Dutov P, Antipova O, Varma S, Orgel J, Schieber JD. Measurement of Elastic Modulus of Collagen Type I Single Fiber. PLoS One. 2016: 11(1): e0145711. DOI: 10.1371/journal.pone.0145711.

  3. Kadler KE, Baldock C, Bella J, Boot-Handford RP. Collagens at a glance. J Cell Sci. 2007;120(12):1955-1962.

  4. Mienaltowski MJ, Birk DE. Structure, physiology, and biochemistry of collagens.Adv Exp Med Biol. 2014;(802):5-29.

  5. Zhang L, Kahn CJ, Chen HQ, Tran N, Wang X. Effect of uniaxial stretching on rat bone mesenchymal stem cell: orientation and expressions of collagen types I and III and tenascin-C. Cell Biol Int. 2008;32(3):344-52.

  6. Chiquet-Ehrismann R. Tenascins. Int J Biochem Cell Biol. 2004; 36(6): 986-90.

  7. Chen S, Peng C, Wei X, Luo D, Lin Y, Yang T, Jin X, Gong L, Li H, Wang K.. Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway. World J Urol. 2017; 35(8):1247-1254.

  8. Zhang YG, Yang Z, Zhang H, Wang C, Liu M, Guo X, Xu P. Effect of negative pressure on human bone marrow mesenchymal stem cells in vitro. Connect Tissue Res. 2010;51(1): 14-21. DOI: 10.3109/03008200902855891.

  9. Yang Z, Yao JF, Xu P, Zhang JB, Zhang YM, Zhu YJ, Qin SQ, Liu L, Liu H, Hou WK, Xu K.Functions and mechanisms of intermittent negative pressure for osteogenesis in human bone marrow mesenchymal stem cells. Mol Med Rep. 2014;9(4):1331-6. DOI: 10.3892/mmr.2014. 1968.

  10. Abbadi OS. Measuring Collagen Concentration in Histology Slides of Mice Skin Using the Adobe® Photoshop® Software. CPQ Medicine. 2019; 6(5): 01-08.

  11. Calvi EN, Nahas FX, Barbosa MV, Calil JA, Ihara SS, Silva Mde S, Franco MF, Ferreira LM. An experimental model for the study of collagen fibers in skeletal muscle. Acta Cir Bras. 2012;27(10):681-6.

  12. “Office of Laboratory animal welfare. Institutional Animal Care and Use Committee Guidebook,” 2nd ed. Bethesda (MD): National Institutes of Health; c 2002.

  13. Abbadi OS, Idris DS, Nafie KM. The histological changes attributed to intermittent negative pressure on mice skin. Chinese J Med Res. 2019; 2(4): 66-69.

  14. Wheeler RM, Wehkamp CA, Stasiak MA, Dixon MA, Rygalov VY. Plants survive rapid decompression: Implications for bioregenerative life support. AdSpR. 2011;47(9):1600-1607. DOI:10.1016/j.asr.2010.12.017

  15. Jönsson KI, Rabbow E, Schill RO, Harms-Ringdahl M, Rettberg P. Tardigrades survive exposure to space in low Earth orbit. Curr Biol. 2008;18(17):R729-R731.

  16. Jönsson KI. Tardigrades as a potential model organism in space research. Astrobiology. 2007;7(5):757-66. DOI: 10.1089/ast.2006.0088.

 To cite this article:

Abbadi OS, Osman AH, Ali NAM.The effect of intermittent negative pressure on collagen concentration in mice skin. Int. J. Med. Lab. Res. 2020, 5(1):20-25

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