International Journal of Medical Laboratory Research (IJMLR)

COMPARISON OF VANCOMYCIN AND HIGH LEVEL AMINOGLYCOSIDE SUSCEPTIBILITIES AND VIRULENCE FACTORS ASSOCIATED WITH BIOFILM OF INFECTION AGENT AND FLORA MEMBER ENTEROCOCCUS STRAINS

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D Bulanik, B Ozer

 

ABSTRACT: Aim: The aim of this study was to investigate and compare virulence factors of infection agent enterococci (IE) and flora member enterococci (FME). Methods: A hundred IE isolated from samples sent to microbiology laboratory and 100 FME isolated from the stools were included in the study. Vancomycin susceptibility was investigated by diffusion gradient method (GDM), high level aminoglycoside resistance and beta-lactamase production were investigated by disk diffusion and nitrocefin disk method respectively. Hemolysin, gelatinase and biofilm production were investigated via phenotypic methods. Results and Conclusions: It was found that hemolysin production rate of IE was more than that of FME. Vancomycin MIC values identified by GDM of biofilm-producing strains was found higher than that of biofilm-free  strains. It was also revealed that moxifloxacin and ampicillin resistance rates of biofilm producing FME and ciprofloxacin, penicilin susceptibility of those that not produce biofilms were higher. Hemolysin production in the infectious was 0,37 times more than those in the FME. Biofilm production in the FME was 3,67 times more than that in IE. It was found out that the virulence factors affected resistance of the strains against some antibiotics .The hemolysin production was more in IE. Biofilm production was more in FME.

 

KEY WORDS: Enterococcus, infectious agent, flora, virulence, slime, hemolysis, gelatinase

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 To cite this article:

Bulanik D, Ozer B. Comparison of vancomycin and high level aminoglycoside susceptibilities and virulence factors associated with biofilm of infection agent and flora member enterococcus strains. Int. J. Med. Lab. Res. 2020; 5,2:1-12. http://doi.org/10.35503/IJMLR.2020.5201