Research Article

      Abstract    

        

HOME

EVALUATION OF A CELL SEPARATOR FOR EFFECTIVENESS IN THE COLLECTION OF APHAERESIS PLATELETS IN A TERTIARY CARE HOSPITAL

Swarupa Bhagwat, Jayashree Sharma, Anand Bodade

 

ABSTRACT: Platelet (PLT) transfusions play a vital role in the management of patients with thrombocytopenia or severely impaired platelet function. Platelet concentrates derived from aphaeresis are preferred in a clinical setting to have lesser alloimmunization rates in patients. There are several aphaeresis machines i.e. cell separators available from different manufacturers that differ in their design, principles, and parameters that ultimately affect the final product. We evaluated an aphaeresis instrument, the Haemonetics MCS + concerning platelet yield, collection efficiency (CE), and collection rate (CR) in a retrospective observational study in 309 donors. The Haemonetics MCS + cell separator efficiently collected apheresis platelets with median PLT yields of 3.63 × 1011, mean CE of 38.12% ± 11.9% and mean CR of 0.059± 0.011 × 1011/min. The median blood volume processed was 2654 ml (1293-3940), and the median volume of acid citrate dextrose-A (ACDA) used in collections on the device was 323 (171-455) ml. Also, this device allowed the collection of white blood cell (WBC) reduced platelet-aphaeresis with mean 0.37± 0.27x106 WBC content. No serious donor or recipient reactions occurred however minimal adverse reactions encountered during procedures and well managed and tolerated by donors without any hesitations for future donations.

 

KEY WORDS: Haemonetics MCS+, Collection rate, collection efficacy, platelet yield

REFERENCES:

  1. [1] Textbook of Modern blood banking and transfusion practices, Denise Harmening 6thedition.

  2. [2] Slichter, S. J. Platelet Transfusion Therapy. Hematology/Oncology Clinics of North America, 2007;21,4, 697–729.

  3. [3] Patel AP, Kaur A, Patel V, Patel N, Shah D, Kanvinde S, Prajapati S, Patel H, Rathod D, Adesara R, Rani S. Comparative study of plateletpheresis using Baxter CS 3000 plus and Haemonetics MCS 3P. Journal of Clinical Apheresis: The Official Journal of the American Society for Apheresis. 2004; 19,3:137-41.

  4. [4] Vavic N, Tomasevic R, Bogdanovic G, et al. Parameters affecting platelet yield in the apheresis platelet concentrates. Vox Sanguinis 2000; 79:277.

  5. [5] Lai C, Pennefather D, Ong L. Comparison for the Different Apheresis Machines for the Final Platelet Products in the Centre for Transfusion Medicine, Singapore. Transfusion. 2002 Sep; 42.

  6. [6] Stiegler G, Leitner G, Panzer S, et al. Comparison of platelet collection efficiency, leukocyte contamination and platelet storage lesion in the MCS plus, revision C2, and the Cobe - Trima. AABB Annual Conference 2004; 272.

  7. [7] Standards For Blood Banks & Blood Transfusion Services, National AIDS Control Organisation Ministry of Health and Family Welfare Government of India New Delhi 2007.

  8. [8] Accreditation standards on blood banks/ blood centers and transfusion services, NABH, Third Edition, June 2016.

  9. [9] Saran RK, editor. Transfusion medicine: technical manual. Directorate General of Health Services, Ministry of Health and Family Welfare, Government of India; 2003.

  10. [10] Effectiveness of Hemonetics MCS cell separator in the collection of apheresis platelets.Kekik et al, Transfusion and Apheresis Science 53 2015; 396–398

  11. [11] Ying H, Bihua Z, Guilan L. Discussing the reasons of 25 apheresis platelets contaminated with overage RBCs. AABB Annual Conference 2004;14.

  12. [12] Me Leod BC, Price TH, Owen H, et al. Frequency of immediate adverse effects associated with apheresis donation. Transfusion 1998; 38 : 938-43.

  13. [13] Raina V, Makroo RN, Goyal N. Adverse effects of platelets pheresis in Asian blood donors.Vox Sang 2002; 83 (Suppl): S89

  14. [14] Margos K, Bellia M, Tsevrenis V, Charalampous P, Andrioti E. Adverse effects on donors and problems during plateletpheresis by using continuous and intermittent flow room separators. Vox Sang 2002; 83 (Suppl) :S88.

  15. [15] Ranganathan S. Comparison of plateletpheresis on the Fresenius AS. TEC 204 and Haemonetics MCS 3p. Journal of Clinical Apheresis: The Official Journal of the American Society for Apheresis. 2007;22,1:1-4.

  16. [16] Salvadori U, Minelli C, Graziotin B, Gentilini I. Single-donor platelet apheresis: observational comparison of the new Haemonetics Universal Platelet protocol with the previous Concentrated Single Donor Platelet protocol. Blood Transfusion. 2014;12,2:220.

  17. [17] Ahmed AS, Leheta O, Younes S. In vitro assessment of platelet storage lesion in leukoreduced random donor platelet concentrates. Blood Transfusion. 2010 ;8,1:28.

  18. [18] Seghatchian J, Beard M, Krailadsiri P. The role of in-process qualification in quality improvement of the haemonetics MCS plus leucodepleted platelet concentrate. Transfusion science. 2000;22,3:165-9.

  19. [19] Seghatchian J. Universal leucodepletion: an overview of some unresolved issues and the highlights of lessons learned. Transfusion and Apheresis Science. 2003;29,2:105-17.

  20. [20] Seghatchian J, Beard MJ, Krailadsiri P. Studies on the improvement of leucodepletion performance of the Haemonetics MCS+ for production of leucodepleted platelet concentrate. Platelets. 2001 ;12,5:298-301.

  21. [21] Burgstaler EA, Pineda AA, Wollan P. Plateletapheresis: comparison of processing times, platelet yields, and white blood cell content with several commonly used systems. Journal of Clinical Apheresis: The Official Journal of the American Society for Apheresis. 1997;12,4:170-8.

  22. [22] Burgstaler EA, Pineda AA, Wollan P. Platelet apheresis: comparison of processing times, platelet yields, and white blood cell content with several commonly used systems. Journal of Clinical Apheresis: The Official Journal of the American Society for Apheresis. 1997;12,4:170-8.

  23. [23] Moog R, Müller N. White cell reduction during plateletpheresis: a comparison of three blood cell separators. Transfusion. 1999 ;39,6:572-7.

  24. [24] Swarup D, Dhot PS, Arora S. Study of single donor platelet (SDP) preparation by Baxter CS 3000 plus and Haemonetics MCS plus. Medical Journal Armed Forces India. 2009 ;65,2:137-40.

  25. [25] Shaikh S, Usman M, Wadood M, Shaikh A (2019) Comparative Analysis of Plateletpheresis Using Different Cell Separators Fenwal Amicus, Fresenius COM.TEC and MCS Plus. J Blood Lymph 9: 247

  26. [26] Chaudhary R, Das SS, Khetan D, Ojha S, Verma S. Comparative study of automated plateletpheresis using five different apheresis systems in a tertiary care hospital. Transfusion and Apheresis Science. 2009;40,2:99-103.

  27. [27] Bueno JL, García F, Castro E, Barea L, González R. A randomized crossover trial comparing three plateletpheresis machines. Transfusion. 2005;45,8:1373-81.

  28. [28] Heba N and Noha BH. Plateletpheresis: A Comparative Study Between Haemonetics MCS Plus and Spectra Trima. Thromb Haemost Res. 2019; 3,1: 1020

  29. [29] Keklik M, Keklik E, Kalan U, Ozer O, Arik F, Sarikoc M. Comparison of Plateletpheresis on the Textbook of Modern blood banking and transfusion practices, Denise Harmening 6thedition.

 To cite this article:

Bodade A, Bhagwat S, Sharma J. Evaluation of a cell separator for effectiveness in the collection of aphaeresis platelets in a tertiary care hospital. Int. J. Med. Lab. Res. 2021; 6,1:28-34. http://doi.org/10.35503/IJMLR.2021.6104