top of page
Technical Notes





A K Jaiswal


ABSTRACT: Thin layer chromatography (TLC) system plays very important role for analysis of several chemical compounds. TLC is a separation technique which is used for separating compounds that are dissolved in solution. It is a technique for identification and purification of mixtures of several components for analytical purposes. Applications are found in diverse fields such as in Forensic Science, Pharmaceuticals, Agriculture, Food and Flavor, Clinical tests and in many others. Standard Operating Procedure (SOP) of Thin layer chromatography has been developed. The different steps involved for operating Thin layer chromatography system have been successfully explained.


KEY WORDS: SOP, Thin layer chromatography, TLC, mobile-phase


  1. Berezkin VG, Mardanov RG, Maliovska I, Rozylo JK. Thin layer chromato- graphy with an isolated support and forced flow of the mobile phase. J Planar Chromatography. 2002;15:377-9.

  2. Campbell A, Chejlana MJ, Sharma J. Use of a modified flatbed scanner for documentation and quantification of thin layer chromatography detected by fluorescence quenching. J Planar Chromatography. 2003;16:244-6.

  3. Carpinella MC, Giorda LM, Ferrayoli CG, Palicos SM. Antifungal effects of different organic extracts from Meliaaze darachL. on phytopathogenic fungi and their isolated active components. JAgric FOOD Chromatography. 2003;51:2506- 11.

  4. Laboratory procedure manual, Forensic Toxicology. Selective and Scientific books Publisher and Distributors;2005.

  5. Marutoiu C, Filip M, Tigae C, Coman V, Gresu R, Marcu G. Synthesis and characterization of alumina R chemically modified with n-octyl for use as a stationary phase in TLC. J Planar Chromatography.2003;16:183-5.

  6. Rack KD,Coats JR. Comparative degradation of organophosphorus insecticide in soil: Specificity of enhanced microbial degradation. J Food Chem. 1990; 36:193-9.

  7. Rack KD, Coats JR. Enhanced Biodegradation of Pesticides in the environment. ACS Symposium series 426, American Chemical Society, Washington DC; 1990.

  8. Reich E, Schibli A. High performance Thin Layer Chromatography for the analysis of medicinal plants. Thieme Medicinal Publishers, Inc. New York; 2006.

  9. Reiffova K,Vicova VP, Orinak A, Florida K, Gondova T. Preliminary TLC analysis of fructoligo saccharides as feed additives. J Planar Chromatography. 2003;16: 52-7.

  10. Sanganalmath PU, Yogaraje CV, Gowtham MD, Nayak VG, Mohan BM. Quantitative Densitometric determination of Quinolphosin Postmortem Blood by HPTLC. IJMT LM.2007;9,2:30-3.

  11. Sethi PD. High Performance Thin Layer Chromatography: Quantitative analysis of pharmaceutical formulation, CBS Publishers and Distributors.

  12. Sharma BK. Instrumental Methods of Chemical Analysis. Krishna Prakashan Media (P) Ltd, Meerut, 16th Edition;1997.

  13. WillardHH.InstrumentalMethodsofAnalysis.6thedition,CBS Publisher sand Distributors, Delhi;1986.

  14. Yagiie C, et al. Multiresidue determination of organochlorine pesticides and polychlorinated biphenyls in milk by Gas Chromatography with electron capture detector after extraction by matrix solid-phase dispersion.AOAC.2001; 84,5:1561-8.

 To cite this article:

Jaiswal AK. Standard operating procedure (sop) for thin layer chromatography (TLC). Int. J. Med. Lab. Res. 2021; 6,2:69-74.

bottom of page