Case Study





Rohini Sharma, Binit Kumar Singh, ChaitenyaVerma, Prahalad Kumar, Pankaj Jorwal

ABSTRACT: Post-operative wound infection, mesh site infection and delayed healing due to Nontuberculous mycobacteria (NTM) is scarcely reported in literature and hence they not recognized as a common cause for these. Generally, wound infections due to atypical mycobacteria shows delayed/non-healing because they do not respond to conventional antibiotics. Rapid identification of rapidly growing mycobacteria (RGM) like M.abscessus can be done by molecular methods using DNA probe hybridization. We report a case of non-healing herniorrhaphy due to wound infection by M. abscessus which was later identified by Line probe assay (CM/AS kit) and treated successfully. This case report emphasizes the importance of comprehensive evaluation of any non-healing postoperative wound by staining, cartridge based nucleic acid amplification test, liquid culture or by any other techniques extensively, in order to detecting an elusive cause such as M.abscessus.

KEYWORDS: Herniorraphy, Nontuberculous mycobacteria ,Atypical mycobacteria




  1. Nag VL, Rahman W, Maurya AK, Kumar M, Kumar, Dhole TN. post-surgical wound due to NTM (Mycobacterium fortuitum) in immunocompetent patient treated by conservatively in tertiary care centre. J Biomed Sci and Res. 2010;2:227–30

  2. Shah AK, GambhirRPS,Hazra N, R Katoch.. mycobacteria in surgical wounds- a rising cause of concern?, Indian J Surg. 2010; 72:206–210 DOI: 10.1007/s12262-010-0057-9

  3. Kalita JB, Rahman H, Baruah KC. Delayed postoperative wound infections due to non-tuberculous  Myco-bacterium. 2005; :535–39. 

  4. Couto I, Machado D, Viveiros M, Rodrigues L, Amaral L. Identification of nontuberculous Mycobacteria in clinical samples using molecular methods: a 3-yearstudy. Clin Microboil Infect. 2010; 16:1161–64. 

  5. García-Agudo L, Jesús I, Rodríguez-Iglesias M, García-Martos P. Evaluation of Inno-lipa Mycobacteria v2 assay for identification of rapidly growing  Mycobacteria. Brazilian Journal of Microbiology. 2011;42:1220–26. 

  6. Caplivski D, Scheld WM. Consultations in infectious diseases: a case based approach to diagnosis and management. New York: Oxford University Press; 2012:274–6

  7. Griffith DE, Aksamit T, Brown-Elliott BA, et al. An official ATS/IDSA statement: diagnosis, treatment, and prevention of non-tuberculous mycobacterial diseases. Am J RespirCrit Care Med 2007;175:367–416

  8. Mandell GL, Bennett EB, Dolin R. Principles and practice of infectious diseases. 7th edn Philadelphia: Churchill Livingstone Elsevier, 2010:3194–95

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