International Journal of Medical Laboratory Research (IJMLR)

STANDARD OPERATING PROCEDURE (SOP) FOR DOUBLE BEAM UV-VIS SPECTROPHOTOMETER UV5704SS

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AK Jaiswal, Akanksha Priya

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ABSTRACT: UV5704SS double beam UV-Vis spectrophotometer is an instrument which is sophistically used to analyze the samples by measuring the absorbance, transmittance or reflection of ultraviolet and visible light. Applications are found in diverse fields such as Pharmaceuticals, Forensic science, environmental science, food industries, material sciences and research purposes. Standard operating procedure of UV double beam UV-Vis spectrophotometer (Model UV 5704SS) has been developed and has been successfully explained.

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Key words: Standard Operating Procedure (SOP), UV-Vis Spectrophotometer etc

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REFERENCES:​​

1. [1]. Wamsley M, Wathudra P, Hu J, Zhang D. Integrating sphere-assisted resonance synchronous spectroscopy for the quantification of material double-beam UV-Vis absorption and scattering extinction. Anal Chem. 2022;94,33:11610–8.

2. [2]. Bessen NP, Bertelsen ER, Shafer JC. Permanganometric titration for the quantification of purified bis(2,4,4-trimethylpentyl) dithiophosphinic acid in n-dodecane. ACS Omega. 2021;6,12:8463–8.

3. [3]. Sekandi P, Namukobe J, Byamukama R, Nagawa CB, Barbini S, Bacher M, et al. Antimicrobial, antioxidant, and sun protection potential of the isolated compounds from Spermacoce princeae (K.Schum). BMC Complement Med Ther. 2023;23,1:201.

4. [4]. Bhattacharjee H, Das D, Bhattacharjee K, Buragohain S, Jaberi H. Transmittance characteristics of transparent hydrophobic acrylic foldable intraocular lenses that were in vivo for a prolonged period of time: A UV-visible spectrophotometric study. Indian J Ophthalmol. 2023;71,12:3663–8.

5. [5]. Ibrahimi D, Aviles M, Valencia Luna G, Rodriguez Resendiz J. Deciphering the physical characteristics of ophthalmic filters used in optometric vision therapy. Healthcare (Basel). 2024;12,21:2177.

6. [6]. Fleming P. Complete theoretical treatment of the transmittance ratio ultraviolet/visible spectrophotometric stray radiant energy test method. Appl Spectrosc. 2009;63,10:1128–33.

7. [7]. Karanasiou C, Dionysopoulos D, Naka O, Strakas D, Tolidis K. Effects of tooth bleaching protocols assisted by Er,Cr:YSGG and diode (980 nm) lasers on color change of resin-based restoratives. J Esthet Restor Dent. 2021;33,8:1210–20.

8. [8]. Memis I, Dionysopoulos D, Papadopoulos C, Mourouzis P, Davidopoulou S, Tolidis K. Effect of air-abrasion pretreatment with three desensitizing agents on efficacy of in-office tooth bleaching. J Esthet Restor Dent. 2021;36,10:1426–36.

9. [9]. Sayyed MI, Almugrin AH, Mahmoud KA, Abouhaswa AS. Influence of increasing SnO₂ content on the mechanical, optical, and gamma-ray shielding characteristics of a lithium-zinc borate glass system. Sci Rep. 2022;12,1:1800.

10. [10]. Palma G, Cozzarini L, Capria E, Fraleoni-Morgera A. A home-made system for IPCE measurement of standard and dye-sensitized solar cells. Rev Sci Instrum. 2015;86,1:013112.

11. [11]. Wei W, Hong R, Jing M, Shao W, Tao C, Zhang D. Thickness-dependent surface plasmon resonance of ITO nanoparticles for ITO/In-Sn bilayer structure. Nanotechnology. 2018;29.1:01575.

12. [12]. Samadi-Maybodi A, Atashbozora E. Quantitative and qualitative studies of silica in different rice samples grown in north of Iran using UV-vis, XRD and IR spectroscopy techniques. Talanta. 2006;70,4:756–60.

13. [13]. Al-Huqail AA, Hatata MM, Al-Huqail AA, Ibrahim MM. Preparation, characterization of silver phyto nanoparticles and their impact on growth potential of Lupinus termis L. seedlings. Saudi J Biol Sci. 2018;25,2:313–9.

14. [14]. Jan MT, Ali A, Uddin K, Said G, Islam MG, Uddin SH, et al. Antimicrobial and antioxidant activities of silver nanoparticles synthesized by novel biogenic method using mixed reductants. Pak J Pharm Sci. 2021;34,3:995–1001.

15. [15]. Surendra TV, Roopan SM, Al-Dhabi NA, Arasu MV, Sarkar G, Suthindhiran K. Vegetable peel waste for the production of ZnO nanoparticles and its toxicological efficiency, antifungal, hemolytic, and antibacterial activities. Nanoscale Res Lett. 2016;11,1:546.

16. [16]. Girgis E, Wahsh MM, Othman AG, Bandhu L, Rao KV. Synthesis, magnetic and optical properties of core/shell Co₁₋ₓZnₓFe₂O₄/SiO₂ nanoparticles. Nanoscale Res Lett. 2011;6,1:460.

17. [17]. Dinc E, Serin C, Tugcu F, Doganay T. Dissolution and assaying of multicomponent tablets by chemometric methods using computer-aided spectrophotometer. Int J Pharm. 2003;250,2:339–50.

18. [18]. Wamsley M, Wathudura P, Hu J, Zhang D. Integrating-sphere-assisted resonance synchronous spectroscopy for the quantification of material double beam UV-Vis absorption and scattering extinction. Anal Chem. 2022;94,33:11610–8.

19. [19]. Verma G, Mishra M. Development and optimization of UV-Vis spectroscopy: a review. World J Pharm Res. 2018;7,11:1170–80.

20. [20]. Purbaningtias TE, Larasati M, Kurniawati P, Wiyantoko B. The performance study of a single and double beam UV-Vis spectrophotometer on nitrite determination in groundwater. AIP Conf Proc. 2020; 2229:030007.

21. [21]. Operating manuals of double beam UV-visible spectrophotometer.