International Journal of Medical Laboratory Research (IJMLR)

JATROPHA TANJORENSIS LEAF EXTRACT EFFECT ON THE EXPRESSION PATTERN OF RUNX1 MRNA AND ETV6 IN AMELIORATING ANAEMIA

 

OE Odaburhine, EMM Oyenmwosa, AA Iroghama

​

ABSTRACT:

Jatropha tanjorensis is a plant that is known to possess various bioactive compounds with potential medicinal properties. Investigating its role in haemopoiesis and the associated molecular pathways could offer useful insights into its therapeutic potential for conditions related to blood cell synthesis and their related disorders. The objective of this study was to determine the effects of aqueous Jatropha tanjorensis leaf extract on RUNX1 mRNA and ETV6 mRNA in mediating haemopoiesis using male Wistar rats. A total of thirty (30) male adult wistar rats were selected into five (5) groups. The groups were control group, phenyl hydrazine group, 1000mg/Kg of Jatropha tanjorensis group, 2000mg/Kg of Jatropha tanjorensis group and 4000mg/Kg of Jatropha tanjorensis group. RNA extraction and semi-quantitative polymerase chain reaction (PCR) was used to isolate RNA from rat bone marrow; complimentary DNA was synthesized and was subsequently amplified using polymerase chain reaction. Gel electrophoresis was used to determine the bands of the genes. The findings revealed that the mRNA expression pattern of RUNX1 was significantly higher (p<0.001) in the untreated,1000mg/kg and 2000mg/kg when compared to the control group. The expression pattern of RUNX1 was significantly higher in 4000mg/kg group when compared to control group(p<0.05). The expression pattern of RUNX1 was significantly higher in groups treated with 1000mg/kg, 2000mg/kg and 4000mg/kg when compared to untreated group (p<0.05). The mRNA expression pattern of ETV6 was significantly lower (p<0.001) in untreated, 1000mg/kg and 4000mg/kg groups when compared to the control group. Among the treated groups, only 2000mg/kg showed a significantly higher (p<0.05) expression pattern of ETV6 when compared to both untreated and 1000mg/kg groups. In conclusion, Jatropha tanjorensis significantly increased RUNX1 and ETV6. These results suggest that Jatropha tanjorensis could influence haemopoiesis by modulating the expression of the key regulating factor.

​

KEY WORDS: Jatropha Tanjorensis, Runx1 Mrna, Etv6, Anaemia

​

​

REFERENCE: 

1. [1]. Iwalewa EO, Adeumi CO, Omisore NOA, Adebanji OA, Azike CK, Adesina AO. Pro-antioxidant Effects and Cytoprotective Potentials of Nine Edible Vegetables in South-West Nigeria. Journal of Medicinal Food. 2005;8(4):539-544.

2. [2]. Olayiwola G, Iwalewa EO, Omobuwajo OR, Adeniyi AA, Verspohi EJ. The Antidiabetic Potential of Jatropha Tanjorensis Leaves. Nigerian Journal of Natural Products and Medicine. 2004; 8:55-58.

3. [3]. Ehimwenma SO, Osagie AU. Phytochemical Screening and Anti-Anaemic Effects of Jatropha Tanjorensis Leaf in Protein Malnourished Rats. Plant Archives. 2007;7(2):509-516.

4. [4]. Schulze H, Shivdasani RA. Mechanisms of Thrombopoiesis. Journal of Thrombosis and Haemostasis: JTH. 2005;3(8):1717–1724.

5. [5]. Machlus KR, Italiano JE. The Incredible Journey: From Megakaryocyte Development to Platelet Formation. The Journal of Cell Biology. 2013;201(6):785–796.

6. [6]. Chang CC, Tsou HK, Chang HH, Chan LY, Zhuo GY, Maeda T, Lin CY. Runx1 Messenger RNA Delivered by Polyplex Nanomicelles Alleviate Spinal Disc Hydration Loss in a Rat Disc Degeneration Model. International Journal of Molecular Sciences. 2022;23(1):565.

7. [7]. Dubey S, Dubey PK, Umeshappa CS, Ghebre YT, Krishnamurthy P. Inhibition of RUNX1 Blocks the Differentiation of Lung Fibroblasts to Myofibroblasts. Journal of Cellular Physiology. 2022;237(4):2169-2182.

8. [8]. Goyama S, Mulloy JC. Molecular Pathogenesis of Core Binding Factor Leukemia: Current Knowledge and Future Prospects. International Journal of Hematology. 2011;94(2):126–133.

9. [9]. Lam K, Zhang DE. RUNX1 and RUNX1-ETO: roles in hematopoiesis and leukemogenesis. Frontiers in Bioscience. 2012;17(4):1120–1139.

10. [10]. Chen CL, Broom DC, Liu Y, de Nooij JC, Li Z, Cen C, Samad OA, Jessell TM, Woolf CJ, Ma Q. Runx1 determines nociceptive sensory neuron phenotype and is required for thermal and neuropathic pain. Neuron. 2006;49(3):365–377.

11. [11]. Yuan H, Liu Y, Zhang J, Dong J, Zhao Z. Transcription factors in megakaryocytes and platelets. Frontiers in Immunology. 2023; 14:1140501.

12. [12]. Hock H, Meade E, Medeiros S, Schindler JW, Valk PJM, Fujiwara Y, Orkin SH. Tel/Etv6 is an essential and selective regulator of adult hematopoietic stem cell survival. Genes & Development. 2004;18(19):2336–2341.

13. [13]. Wang Q, Stacy T, Binder M, Marin-Padilla M, Sharpe AH, Speck NA. Disruption of the Cbfa2 Gene causes Necrosis and Hemorrhaging in the Central Nervous System and Blocks Definitive Hematopoiesis. Proceedings of the National Academy of Sciences of the United States of America. 1996;93(8):3444–3449.

14. [14]. Idu M, Igbafe G, Erhabor J. Anti-anaemic Activity of Jatropha tanjorensis Ellis and Saroja in Rabbits. Journal of Medicinal Plants. 2014;2(1).

15. [15]. Osayande EA, Ihemeje VI, Osime EO. Jatropha Tranjorensis Leaf Extract Modulation on Glycoprotien IX, 1B Alpha and 1B Beta in Albino Wistar Rats. Sokoto Journal of Medical Medical Laborarory Science. 2025;10(1):20-28.

16. [16]. Lu C, Yang Z, Yu D, Lin J, Cai W. RUNX1 Regulates TGF-β Induced Migration and EMT in Colorectal Cancer. Pathology-Research and Practice. 2020;216(11):153142.

17. [17]. Palanichamy JK, Sharma G, Nidhi T, Chopra A, Bakhshi S. Role of IGF2BP1 and Target Genes in ETV6-RUNX1 Positive B-Acute Lymphoblastic Leukemia. Blood. 2019; 134:3818.

18. [18]. Mayumi A, Yoshida H, Mitsuno K, Nishida N, Osone S, Yasuda T, Imamura T. Leukemic Cells Expressing ETV6-Frk Identified in a Refractory B-ALL Patient Are Sensitive to Dasatinib in Vitro. Blood. 2021; 138:4335.