International Journal of Medical Laboratory Research (IJMLR)

STANDARD OPERATING PROCEDURE (SOP) OF – HIGH PERFORMANCE LIQUID CHROMATOGRAPHY SYSTEM (HPLC)

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Dr. A.K. Jaiswal,Pallavi Choudhary,Shivani Tyagi, Dr. M.Gupt

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ABSTRACT: HPLC is a form of liquid chromatography used for separating compounds that are dissolved in solution. It is a technique for identification, quantification and purification of mixtures for analytical purposes. Applications are found in diverse fields such as in forensic science, pharmaceuticals, food and flavor, clinical tests and in many others. Standard Operating Procedure of HPLC System (Make: DIONEX, Model: ULTIMATE – 3000) has been developed. The different steps involved for operating HPLC System have been successfully explained.

KEYWORDS: Standard Operating Procedure (SOP), HPLC, DIONEX ULTIMATE-3000, blood, urine, pharmaceutical preparations, etc.

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